Localization and clinical significance of kindlin-1 in human gingival epithelia

doi:10.3969/j.issn.0529-1356.2013.05.011

Abstract

Abstract:

Objectiv To investigate distributions of kindlin-1 in human normal gingival epithelia and chronic inflammatory gingival epithelia and establish clinical relevance between kindlin-1 and periodontal diseases.Methods Immunohistochemistry and Western blotting techniques were applied to detect the localization and expression of kindlin-1 of 10 human normal gingival epithelia and 8 chronic inflammatory gingival epithelia. Results Kindlin-1 was mainly localized in basal layer cells and upper granular layer cells of normal gingival epithelia, especially highly expressed in cytoplasm in granular layer cells, with some diffuse staining in the nuclei of suprabasal layer cells. In addition, the higher immunoractivity for kindlin-1 was observed in interdental gingiva than in free marginal gingiva (P<0.05). Compared to normal gingival epithelia, kindlin-1 presented much wider and higher expression in chronic inflammatory gingival epithelia (P<0.05). Western blotting further verified that kindlin-1 in chronic inflammatory gingival epithelia was higher expressed than in normal gingival epithelia (P<0.05). Conclusion The localization and expression of kindlin-1 in human gingival epithelia are closely associated with the differentiation and inflammation.

Key words: Kindlin-1, Gingival epithelia, Immunohistochemistry, Western blotting, Human

XIE Ting-ting MEI Fang ZHONG Jin-sheng TANG Yan ZHANG Hong-quan*. Localization and clinical significance of kindlin-1 in human gingival epithelia[J]. AAS, 2013, 44(5 ): 641-645.

References

［1］ Jiang LY. Pathogenesis and therapy progress of periodontal disease［J］. Modern Medicine and Health, 2010, 26(14):2167-2169. (in Chinese)
姜丽艳. 牙周病的病因与治疗进展［J］. 现代医药卫生, 2010, (14): 2167-2169. 
［2］Wiebe CB, Silver JG, Larjava HS. Early-onset periodontitis associated with Weary-Kindler syndrome: a case report［J］. J Periodontol, 1996, 67(10): 1004-1010.
［3］Techanukul T, Sethuraman G, Zlotogorski A, et al. Novel and recurrent FERMT1 gene mutations in Kindler syndrome［J］. Acta Derm Venereol, 2011, 91(3): 267-270.
［4］Kloeker S, Major MB, Calderwood DA, et al. The Kindler syndrome protein is regulated by transforming growth factor-beta and involved in integrin-mediated adhesion［J］. J Biol Chem, 2004, 279(8): 6824-6833.
［5］Petricca G, Leppilampi M, Jiang G, et al. Localization and potential function of kindlin-1 in periodontal tissues［J］. Eur J Oral Sci, 2009, 117(5): 518-527.
［6］Kahner BN, Kato H, Banno A, et al. Kindlins, integrin activation and the regulation of talin recruitment to alphaIIbbeta3［J］. PLoS One, 2012, 7(3): e34056.
［7］Larjava H, Plow EF, Wu C. Kindlins: essential regulators of integrin signalling and cell-matrix adhesion［J］. EMBO Rep, 2008, 9(12): 1203-1208.
［8］Herz C, Aumailley M, Schulte C, et al. Kindlin-1 is a phosphoprotein involved in regulation of polarity, proliferation, and motility of epidermal keratinocytes［J］. J Biol Chem, 2006, 281(47): 36082-36090.
［9］Harburger DS, Bouaouina M, Calderwood DA. Kindlin-1 and-2 directly bind the C-terminal region of beta integrin cytoplasmic tails and exert integrin-specific activation effects［J］. J Biol Chem, 2009, 284(17): 11485-11497.
［10］Ussar S, Wang HV, Linder S, et al. The Kindlins: subcellular localization and expression during murine development［J］. Exp Cell Res, 2006, 312(16): 3142-3151.
［11］Wato K, Hara T, Yamana K, et al. An insight into the role of barrier related skin proteins［J］. Int J Pharm, 2012, 427(2): 293-298.
［12］Kern JS, Herz C, Haan E, et al. Chronic colitis due to an epithelial barrier defect: the role of kindlin-1 isoforms［J］. J Pathol, 2007, 213(4): 462-470.
［13］Qu H, Wen T, Pesch M, et al. Partial loss of epithelial phenotype in kindlin-1-deficient keratinocytes［J］. Am J Pathol, 2012, 180(4): 1581-1592.
［14］Heinemann A, He Y, Zimina E, et al. Induction of phenotype modifying cytokines by FERMT1 mutations［J］. Hum Mutat, 2011, 32(4): 397-406.
［15］Pan XD, Chen XCh, Zhu YG, et al. Effect of inflammatory responses in microglia induced by oligomeric β-amyloid_1-42 on neuronal cells［J］. Acta Anatomica Sinica, 2008, 39(6): 804-809. (in Chinese )
潘晓东, 陈晓春, 朱元贵, 等. 寡聚态β-淀粉样肽1～42可通过胶质-炎症反应损伤神经元［J］. 解剖学报, 2008, 39(6): 804-809. 
［16］Sin S, Bonin F, Petit V, et al. Role of the focal adhesion protein kindlin-1 in breast cancer growth and lung metastasis［J］. J Natl Cancer Inst, 2011, 103(17): 1323-1337.
［17］Wada M, Masuda K, Tsuruta D, et al. Case of Kindler syndrome resulting from mutation in the FERMT1 gene［J］. J Dermatol, 2012.
［18］Lai-Cheong JE, Moss C, Parsons M, et al. Revertant mosaicism in Kindler syndrome［J］. J Invest Dermatol, 2012, 132(31): 730-732.
［19］Larjava H, Koivisto L, Hakkinen L, et al. Epithelial integrins with special reference to oral epithelia［J］. J Dent Res, 2011, 90(12): 1367-1376.

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